3D PIGMENTSkin
A highly predictive tool to identify and evaluate skin pigmentation modulators
![](/lib/picts/pages1b/3d-pigmented-skin-vignette.jpg)
What is it About?
The melanin pigment system in human skin is extraordinarily well
developed and assures the photoprotection of the skin against
harmful solar radiation.
Specific cell-cell interactions between melanocytes, keratinocytes
and fibroblasts play a fundamental role in the regulation of
melanogenesis and melanin pigmentation.
To identify and evaluate skin pigmentation modulators, LabSkin
Creations team developed a unique and predictive pigmented
reconstructed skin reproducing the three-dimensional architecture
of the melanocyte environment and the interactions of melanocytes
with its cellular partners, keratinocytes and fibroblasts.
3D PIGMENTSkin, a functional full-thickness pigmented skin model
By using our proprietary LabSkin matriX® technology of tissue reconstruction, we developed 3D PIGMENTSkin, a human full-thickness skin model including the pigmentary system.
Not Pigmented
3D model
![Not Pigmented<br />3D model](/lib/picts/pages2/1.-Unpigmented_rond.jpg)
Pigmented
3D model
![Not Pigmented<br />3D model](/lib/picts/pages2/2.-Pigmented_rond.jpg)
3D PIGMENTSkin exhibits a correct integration of melanocytes within the epidermal basal layer showing a constitutive production and transfer of melanin pigments into keratinocytes.
UV-unexposed
![UV-unexposed](/lib/picts/pages2/2.-Pigmented_rond.jpg)
UV-exposed
![UV-unexposed](/lib/picts/pages2/3.-UV-induced_rond.jpg)
The application of ultraviolet (UV) light doses on our 3D PIGMENTSkin leads to a marked and clear increase of the constitutive pigmentation showing its functional state, whereas the number of melanocytes is not changed.
UV-Exposed
Untreated
![UV-Exposed<br />Untreated](/lib/picts/pages2/pigmented-3.jpg)
UV-Exposed
+ Whitening
![UV-Exposed<br />Untreated](/lib/picts/pages2/4.-UV--ttt-copy-2_rond.jpg)
Treatment of 3D PIGMENTSkin stimulated by UV with whitening products of reference results in a strong reduction of the pigmentation and inhibition of melanin transfer.
A responsive model to stress
LIPID PEROXYDATION
UV-unexposed
Untreated
![5. MDA_unexposed](/lib/picts/pages3/5.-MDA_unexposed.jpg)
UV-exposed
Untreated
![6. MDA_UV exposed](/lib/picts/pages3/6.-MDA_UV-exposed.jpg)
UV-exposed
Active-treated
![7. MDA_UV ttt](/lib/picts/pages3/7.-MDA_UV-ttt.jpg)
UV irradiations of 3D PIGMENTSkin model results in oxidative stress with an increase in malondialdehyde (MDA) expression reflecting lipids oxidation, which can be reversed by treatment with antioxidant active.
PROTEIN CARBONYLATION
![](/lib/picts/pages1b/Carbonyl-score_new300.png)
Protein carbonylation, one of the most harmful oxidative protein modifications, is
considered as a major hallmark of oxidative stress-related disorders. After UV irradiations, 3D PIGMENTSkin exhibits a significant increase in oxidized proteins. This protein oxidation can be prevented by treating our 3D skin pigmented model with antioxidant active.
![](/lib/picts/pages1b/carbonyl-chart_new.png)
3D PIGMENTSkin is an advanced and highly predictive model to validate the whitening potential of cosmetics active candidates with a presumed function in melanin synthesis or transfer. LabSkin pigmented full-thickness model including a functional dermis represents also a highly relevant tool to study the fundamental role of cell-cell as well as mesenchymalepithelial interactions in the control of skin pigmentation.